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Why does growth of ETPUM in Medium #$ require oxygen? think about this in terms of how ETPUM can generate a net gain in ATP by processing polyurethane. Remember that the degradation of polyurethane by polyurethane does not expend ATP. In order to answer this question, address each of the following questions in your answer: a. Is there a net gain or loss of ATP during the transport of the citrate? b. Consider the ATPs that can be generated via substrate-level phosphorylation. Will glycolysis be useful for generating any ATPs during growth on polyurethane? How many ATPs can be generated via TCA? Is this enough to support growth (is there a net positive in the ATP tally)? c. Now consider how else ETPUM can generate ATPs (if not by substrate- level phosphorylation). Can this process generate a net positive in the ATP tally? d. Now explain the importance of oxygen as relates to the ATP tally.

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