Bacterial Conjugation Lab Summary Questions: 1. What

Bacterial Conjugation Lab Summary Questions: 1. What were the strains of bacteria and their genes? 2. What were the two antibiotics used? 3. Under normal circumstances, what affect would the antibiotics have on the bacteria if they did not have the plasmids? 4. List the bacteria, and where the genes are carried. Lab Results First inoculation: Explain the results of the following plates. Each strain must be explained on each plate. Explain your responses. LB agar (plain) – growth on both sides LB agar +strep – growth on strain 1. No growth on strain 2. LB agar + amp – no growth on strain 1. Growth on strain 2. LB agar + strep + amp – no growth on strain 1. No growth on strain 2. Mating Plate – Explain why there was growth on the mating plate. After the mating plate – Explain why there was growth in each plate. Include what happened in the bacteria to produce the growth on all plates. Post lab questions 1. Based on the growth response to the various antibiotics, have conjugation and transfer of DNA (recombination) occurred? Explain your response. 2. Can you tell whether the growth on the mating plate consists of recombinant cells only or whether it merely contains cells of strains 1 and II? Explain your response. 3. On the basis of the results of this study, can one determine whether the STR gen from Strain I was transferred to Strain II or whether the AMP gene on the plasmid in Strain II was transferred into Strain I. Explain your response. SCIENCE HEALTH SCIENCE NURSING BIOMED 101

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