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ANSWER THE FOLLOWING QUESTIONS BASED QUALITATIVE TEST FOR SUGARS LABORATORY FOR SELIWANOFF’S TEST AND ORCINOL TEST. MAKE SURE TO WRITE OBSERVATION AND COLOR REACTION OF THE SOLUTION AND, TAKE NOTE THE LENGTH OF TIME IN THE FORMATION OF PRECIPITATE.
A. SELIWANOFF’S TEST
1. Use three clean and dry test tubes and label as follows:
Test tube 1 – place 10 drops of 1% glucose
Test tube 2- place 10 drops of 1% fructose
Test tube 3- place 10 drops of 1% sucrose
2. Add 2mL of Seliwanoff’s reagent in each test tube. Immerse the tube at the same time in a water bath of boiling water. Note the length of time in the formation of a precipitate.
QUESTIONS:
Write the observation and color reaction on the following solution based on the procedure above. Make sure to note the length of time in the formation of precipitate. Explain your answers.
1. GLUCOSE
2. FRUCTOSE
3. SUCROSE
B. ORCINOL TEST (BIAL’ S TEST)
1. Use three clean and dry test tubes and label as follows:
Test tube 1 – place 10 drops of 1% glucose
Test tube 2- place 10 drops of 1% fructose
Test tube 3- place 10 drops of 1% ribose
2. Add 2mL of Bial’s reagent in each test tube. Immerse the tube at the same time in a water bath of boiling water. Note the color of the solution or the formation of a precipitate.
QUESTIONS:
Write the observation and color reaction on the following solution based on the procedure above. Make sure to note the length of time in the formation of precipitate. Explain your answers.
1. GLUCOSE
2. FRUCTOSE
3. RIBOSE

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