TRIGLYCERIDES DETERMINATION Fats contain a significant part

TRIGLYCERIDES DETERMINATION Fats contain a significant part of the diet in many individuals. On the average, there are about 60g to 130g of fat ingested, absorbed and transported in the body, mostly in the form of triglycerides. Triglycerides is metabolized in the liver into more polar compounds as monoglycerides, diglycerides and free fatty acids. Fatty acids are stored in cells as if triglycerides begins with the activation of fatty acids to acyl-CoA ester. There are different routes involved in the formation of triglycerides. Together with total cholesterol, triglycerides determination are of interest in the diagnosis and treatment of atherosclerosis, poorly controlled diabetes mellitus, nephrosis and liver disease, and other disease involving lipid metabolism. A variety of methods have been used to measure plasma triglycerides. There are the stepwise colorimetric methods based on the Van Handel-Zilversmit reaction and Hantzsch reaction. The CDC reference method used a chloroform extraction procedure followed by silicic aci chromatography to isolate the triglycerides, then apply the Zilversmit reaction to measure the triglycerides concentration. But the methos most commonly used for clinical or epidemiologic purposes are based on the hydrolysis of triglycerides and the measurement of glycerol that is released in the reaction. Questions for Research: 1. Describe the metabolism of triglycerides and the lipoprotein s associated (chylomicrons and. VLDL). 2. What are the functions of triglycerides in the body? 3. What are the steps involved in the colorimetric methods used in the determination of triglycerides. 4. Describe the colorimetric reaction principles of: a. Van Handel and Zilversmit b. Hantzsch 5. Give other enzymatic methods (other appproches) to the triglycerides assay. 6. Describe the plasma standing test. What is the importance of this test? 7. Give several other factors as sources of variation affecting triglycerides assay 8. Discuss the clinical significance associated with abnormal levels of triglycerides. Notes: Please kindly make the answer accurate, informative and precise. Include the references.

******CLICK ORDER NOW BELOW AND OUR WRITERS WILL WRITE AN ANSWER TO THIS ASSIGNMENT OR ANY OTHER ASSIGNMENT, DISCUSSION, ESSAY, HOMEWORK OR QUESTION YOU MAY HAVE. OUR PAPERS ARE PLAGIARISM FREE*******."